Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody Outstanding specificity with no cross-reactivity or background.Compatible with most blue-stained ladders.Develop your marker directly on X-ray film.
#Nuclear loading control western blot manual
Faster and easier protein detection - manual marking is no longer necessary.This makes the positioning of the marker and thus the accurate detection of proteins significantly easier. Diagenode has developed a revolutionary new antibody that specifically reacts with this blue dye, enabling direct visualization of the different marker fragments on the blot. Most prestained protein MW markers used in western blot contain fragments that are labelled with a blue dye. Learn more about this antibody Blue ladder-HRP monoclonal antibody Visualize your marker directly on film To check that the signal detection after antibody incubation is homogenous across the lines.To confirm that the electrotransfer was done equally across the lines.To verify that equal sample amount was loaded on the gel.The marker (in kDa) is shown on the left,and the position of the protein of interest is indicated on the right. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Western blot was performed on whole cell extracts (30 μg) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using the monoclonal antibody against H3. Western blot analysis using H3pan monoclonal antibody
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